| Lane/Order | BIB | ATHLETE | Seeding Time | Note | |
|---|---|---|---|---|---|
| 1 | 401 | HELP | CHAN TONG FEI | 27.93 | q |
| 2 | 502 | UTM | ASHLEY NATASHA HENDRICKS | 28.42 | q |
| 3 | 417 | POLI | NIA JOVIC SUAN | 26.97 | Q |
| 4 | 473 | UPSI | MIS`EZATUL SHAFIEQA BINTI SAPAWI | 26.99 | Q |
| 5 | 471 | UPSI | NURUL WARDATUL HUWAIDA BINTI MOHD HAMKA | 26.16 | Q |
| 6 | 406 | KKMAS | DANEA HUMAIRAH BINTI SHARYMAN SUFRY | 27.15 | Q |
| 7 | 441 | UNIMAS | EVELYNE TAN XIE YIE | 27.25 | Q |
| 8 | 416 | POLI | BIBI HALINA BINTI ALOYSIUS | 27.76 | Q |