| Lane/Order | BIB | ATHLETE | Seeding Time | Note | |
|---|---|---|---|---|---|
| 1 | 159 | UIAM | MUHAMMAD FATHAN MUTTAQIN BIN KHAIRUL `AZMI | 54.10 | q |
| 2 | 110 | KLUST | RIVAIENESH NAIKKER | 53.81 | Q |
| 3 | 210 | UNIMAS | DEXTER PALMIN | 53.54 | Q |
| 4 | 125 | POLI | IAN JOVIC SUAN | 51.05 | Q |
| 5 | 149 | UIAM | AHMAD HAZIQ BIN ZURAIK | 51.34 | Q |
| 6 | 344 | UNITEN | MUHAMMAD NUR HARRIS BIN AZHAR | 51.47 | Q |
| 7 | 167 | UKM | FARISH NAZMI BIN ISHAK | 53.09 | Q |
| 8 | 328 | UUM | MOHD ADLI BIN ARSHAD | 53.66 | q |