| Lane/Order | BIB | ATHLETE | Seeding Time | Note | |
|---|---|---|---|---|---|
| 1 | 167 | UKM | FARISH NAZMI BIN ISHAK | 60.22 | q |
| 2 | 328 | UUM | MOHD ADLI BIN ARSHAD | 58.40 | q |
| 3 | 213 | UNIMAS | TAN WEI WEN | 58.39 | Q |
| 4 | 238 | UPM | MUHAMMAD FAKRUL AFIZUL BIN NASIR | 55.35 | Q |
| 5 | 271 | UPSI | MUHAMMAD ADIB IRFAN BIN AZIZAN | 56.15 | Q |
| 6 | 232 | UPM | MOHAMMAD RAMDHANI AZANI BIN ROSLIE | 56.35 | Q |
| 7 | 286 | UPSI | WAN MOHAMAD ALHAFIZ BIN WAN MOHAMED KOZANI | 57.99 | Q |
| 8 | 210 | UNIMAS | DEXTER PALMIN | 61.00 | Q |