| Lane/Order | BIB | ATHLETE | Seeding Time | Note | |
|---|---|---|---|---|---|
| 1 | 047 | KBN | MOHAMMAD HAQEEM ALIMIN SABRI | 59.75 | q |
| 2 | 219 | KMU | DANIEL SHANEN BIN MACLIN | 59.99 | q |
| 3 | 181 | KKU | JARREL SHAMRY | 59.44 | q |
| 4 | 624 | SDK | DEXTER PALMIN | 58.23 | Q |
| 5 | 715 | TBN | HEDDON TANGAR | 58.23 | Q |
| 6 | 175 | KKU | MUHAMMAD NURMANSYAH BIN NURDIN | 55.88 | Q |
| 7 | 465 | PPG | AWANG MUHAMAD SYAHRIZAN BIN AG KASSIM | 59.43 | Q |
| 8 | 350 | LDU | ALVINSYAH ZIQKRYZAM BIN PAUL | 58.25 | q |