| Lane/Order | BIB | ATHLETE | Seeding Time | Note | |
|---|---|---|---|---|---|
| 1 | 1638 | POAT | SHAZRIL ASHRAFF BIN SHAMSURBAHRY | 58.54 | q |
| 2 | 469 | NEGERI SEMBILAN | MOHAMAD AMRIE BIN MOHD ZABHAR | 58.23 | q |
| 3 | 9 | SINGAPORE SPORTS SCHOOL (SSP) | VEROY CHUA JIA HAO | 57.81 | q |
| 4 | 781 | SUKMA JOHOR | MOHAMAD FIQRY BIN MOHD HAIRIL | 56.57 | q |
| 5 | 413 | SUKMA PERAK | MOHAMMAD ADIB IRFAN BIN AZIZAN | 56.62 | q |
| 6 | 1658 | POAT | NIK ABRISAM JAZMI BIN NIK ADZLI | 57.53 | q |
| 7 | 1092 | PULAU PINANG | MUHAMMAD ABSHAR DANISH B. MOHD AZZRUL | 57.70 | q |
| 8 | 767 | SUKMA JOHOR | MUHAMMAD DANISH NABIL B. KAMARUDIN | 58.01 | q |