| Lane/Order | BIB | ATHLETE | Seeding Time | Note | |
|---|---|---|---|---|---|
| 1 | 1025 | WIJUARA | TAN KAR KIEN | 51.22 | q |
| 2 | 628 | SSTMI | ARYAN RAFIQUE BIN ROMAFITRI | 51.20 | q |
| 3 | 1094 | PULAU PINANG | VENKET PRABU A/L KALITHASAN | 50.57 | q |
| 4 | 1091 | PULAU PINANG | MUHAMMAD SAYYID AMIN BIN ROSLAN | 50.11 | q |
| 5 | 510 | MSN SELANGOR | MUHAMMAD DANIEL HAQIEM BIN AZMAN | 50.29 | q |
| 6 | 1095 | PULAU PINANG | DARWISH RUSHDAN BIN MOHAMAD FIRDAUS | 50.31 | q |
| 7 | 774 | SUKMA JOHOR | MUHAMMAD AZZAM DARWISY BIN SHAMSUL | 50.51 | q |
| 8 | 196 | MUTIARA IMPIAN SPINTER | deniswar A/L yanasegran | 51.01 | q |