| Lane/Order | BIB | ATHLETE | Seeding Time | Note | |
|---|---|---|---|---|---|
| 1 | 118 | MALAYSIA | LANA ALEXA JOSE LIM | 59.26 | q |
| 2 | 119 | MALAYSIA | SHAYNA MARIANNE PEREIRA JESINDRAN | 58.87 | q |
| 3 | 606 | MYANMAR | MA ZU HLAING MOE | 58.52 | Q |
| 4 | 521 | PHILIPPINES | JERALYN RODRIGUEZ | 55.71 | Q |
| 5 | 912 | VIETNAM | THI HUONG DONG | 57.87 | Q |
| 6 | 406 | THAILAND | PHICHAYAPHA SUKAUE | 58.13 | Q |
| 7 | 407 | THAILAND | WARINTHON DONAIN | 58.19 | Q |
| 8 | 203 | INDONESIA | DANCELINA WELLY TAURUY | 59.08 | Q |