| Lane/Order | BIB | ATHLETE | Seeding Time | Note | |
|---|---|---|---|---|---|
| 1 | 501 | PHI | MICO VILLARAN | 55.77 | q |
| 2 | 425 | THA | SARAYU KAEONU | 55.25 | q |
| 3 | 108 | MAS | EMMANUEL MUPHY JOSBERT | 54.81 | Q |
| 4 | 508 | PHI | IVAN CABANDA | 52.98 | Q |
| 5 | 902 | VIE | NGOC MINH QUACH | 53.68 | Q |
| 6 | 218 | INA | ASYRAFI KHAIZUR YUSQI | 54.59 | Q |
| 7 | 315 | SGP | DARYEN XIN TZE KO | 54.65 | Q |
| 8 | 104 | MAS | MUHAMAD QARLIFF KUYT BIN ABDULLAH | 55.10 | Q |